Ultrasensitive fluorescent proteins for imaging neuronal activity

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Photocontrollable fluorescent proteins for superresolution imaging.

Superresolution fluorescence microscopy permits the study of biological processes at scales small enough to visualize fine subcellular structures that are unresolvable by traditional diffraction-limited light microscopy. Many superresolution techniques, including those applicable to live cell imaging, utilize genetically encoded photocontrollable fluorescent proteins. The fluorescence of these ...

متن کامل

Smart fluorescent probes for imaging macrophage activity.

Macrophages are multi-functional immune cells with key roles in host defense and tissue remodelling. The broad array of macrophage functions has prompted the development of very diverse smart fluorescent architectures, rationally designed to elicit a fluorescent signal only after target engagement. This tutorial review covers recent advances in the design, synthesis and application of smart flu...

متن کامل

Imaging intracellular fluorescent proteins at nanometer resolution.

We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method--termed photoactiv...

متن کامل

Fluorescence lifetime imaging of coral fluorescent proteins.

Corals, like many other coelenterates, contain fluorescent pigments that show considerable homology with the well known green fluorescent protein of the jellyfish Aequoria. In corals, unlike jellyfish, multiple proteins are present and the range of excitations and emissions suggest the possibility of energy transfer. The occurrence of Förster resonant energy transfer (FRET) between fluorescent ...

متن کامل

Fluorescent proteins for multiphoton and super-resolution imaging

87 Progress in fluorescence imaging is enabling the study of biological events at unprecedented detail, thanks to novel microscopy techniques that provide imaging with nanoscale resolution. 3D imaging of living cells within intact tissues, organs, and whole animals, is accessible through multiphoton excitation. The remarkably rich photophysics of Fluorescent Proteins (FPs) makes them flexible e...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nature

سال: 2013

ISSN: 0028-0836,1476-4687

DOI: 10.1038/nature12354